Extraction, purification, physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L. seed

In this study, a novel polysaccharide fraction from Ocimum album seed was extracted and then purified by Cellulose DEAE-52 Sephadex G-200 anion exchange chromatography. The structural, physicochemical antioxidant properties of the main (OAP-1A) were evaluated. contained 94.3% carbohydrate, 3.56% moisture 2.14% ash result gel permeation chromatography (GPC) showed average molecular weight 593 kDa. results high-performance liquid (HPLC) that OAP-1A neutral hetero-polysaccharide composed mannose (35.7%), glucose (33.32%), galactose (19.6%) rhamnose (11.38%). addition, GC–MS data, nuclear magnetic resonance (NMR) spectrum Fourier transform infrared (FT-IR) analysis revealed backbone consists →3)-β-D-Manp-(1→, →3,4)-β-D-Manp-(1→, →3,6)-β-D-Manp-(1→, →3)-α-D-Glcp-(1→, →6)-β-D-Galp-(1→, →4)-α-L-Rhap-(1→ α-D-Glcp-(1→. X-ray diffraction (XRD) semi-crystalline structure in OAP-1A. Differential scanning colorimeter (DSC) thermo-gravimetry (TGA) indicated had relatively high thermal stability. Moreover, strong scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals.